Antisense Inhibition of Tomato Fruit Sucrose Synthase Decreases Fruit Setting and the Sucrose Unloading Capacity of Young Fruit

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Antisense inhibition of tomato fruit sucrose synthase decreases fruit setting and the sucrose unloading capacity of young fruit.

The role of sucrose synthase (SuSy) in tomato fruit was studied in transgenic tomato (Lycopersicon esculentum) plants expressing an antisense fragment of fruit-specific SuSy RNA (TOMSSF) under the control of the cauliflower mosaic virus 35S promoter. Constitutive expression of the antisense RNA markedly inhibited SuSy activity in flowers and fruit pericarp tissues. However, inhibition was only ...

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Isolation and sequencing of tomato fruit sucrose synthase cDNA.

Suc synthase (EC 2. 4. 1. 13) catalyzes the reversible reaction: Suc + UDP c-, UDP-Glc + Fru. In plants, its main physiological function is to cleave Suc and generate UDPGlc, which is used in such diverse pathways as starch synthesis, cell wall component synthesis, and energy generation (Huber and Akazawa, 1986; Sung et al., 1988). It has been demonstrated that Suc synthase is a key enzyme duri...

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Sucrose Synthase, Starch Accumulation, and Tomato Fruit Sink Strength.

Contrasting evidence has accumulated regarding the role of acid invertase and sucrose synthase in tomato fruit sink establishment and maintenance. In this work the relationships among the activities of sucrose synthase and acid invertase, Lycopersicon esculentum Mill cv UC-82B fruit growth, and starch accumulation were analyzed in fruit at 0 to 39 d after anthesis. Sucrose synthase, but not aci...

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Sucrose phloem unloading follows an apoplastic pathway with high sucrose synthase in Actinidia fruit.

Phloem unloading plays a pivotal role in photoassimilate partitioning and the accumulation of sugars in sink organs, e.g. fruit. Here, we investigated the pathway of sucrose unloading in kiwifruit (Actinidia deliciasa cv. Qinmei) using a combination of electron microscopy, transport of the phloem-mobile symplastic tracer carboxyfluorescein and enzyme activity and gene expression assays. Our str...

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Sucrose synthase and the fruit of its labor.

Evolutionary selective pressures have clearly induced animals and plants alike to place a premium on liberal locomotive abilities. Because the root systems of plants define a sedentary existence, however, angiosperms in particular invest significant energies in exploiting the mobility of animals. Such exploitation, in fact, is one of the most fascinating evolutionary trends to have marked the b...

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ژورنال

عنوان ژورنال: The Plant Cell

سال: 1999

ISSN: 1040-4651,1532-298X

DOI: 10.1105/tpc.11.12.2407